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Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific <t>ipo13b</t> mutants at 5 dpf labelled using the
Ipo13b Sgrna2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific ipo13b mutants at 5 dpf labelled using the

Journal: Nature communications

Article Title: Importin 13-dependent axon diameter growth regulates conduction speeds along myelinated CNS axons.

doi: 10.1038/s41467-024-45908-6

Figure Lengend Snippet: Fig. 7 | Reduced axon diameter growth does not change myelin thickness along the Mauthner axon. A Representative time course from 3 to 5 dpf depicting the growth in diameter of a Mauthner axon (magenta—Tg(hspGFF62A:Gal4); Tg(UAS:mem-Scarlet)) and growth of myelin in thickness (green—Tg(mbp:eGFP- CAAX)) at somite 15. B Schematic representation of how the g-ratio for an axon is calculated. C–E Quantification of axon diameter (C), myelin thickness (D) and g-ratio (E) for control Mauthner axons followed from 3 to 5 dpf (RM one-way ANOVA with Tukey’s multiple comparison test, n = 18 axons from individual animals with repeated measures at each time point. p < 0.0001 for (C) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), p < 0.0001 for (D) (3 dpf vs. 4 dpf and 4 dpf vs. 5 dpf), and p = 0.0394 for (E) (3 dpf vs. 5 dpf). F Quantification of the percentage of the Mauthner axon myelinated at 5 dpf (two-tailed unpaired t-test, n = 3 axons from individual animals for each genotype, p = 0.9239). G Representative images of the Mauthner axon and its myelin in control and neuron-specific ipo13b mutants at 5 dpf labelled using the

Article Snippet: The annealed oligos were then ligated into U6 promotor-based cassettes: ipo13b sgRNA1 into pU6a:sgRNA#1 (Addgene #64245), ipo13b sgRNA2 into pU6a:sgRNA#2 (Addgene #64246), and ipo13b sgRNA3 into pU6b:sgRNA#3 (Addgene #64247).

Techniques: Control, Comparison, Two Tailed Test